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摘要: |
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为获得雷公藤试管苗生根的最佳条件，以雷公藤试管苗生根率为评价指标，通过对基本培养基MS以及激素ABT1#和IBA的浓度等因子进行二次正交旋转组合试验，确定雷公藤试管苗生根的最佳培养基组成为1/2MS 0.6 mg?L-1 ABT1# 0.2 mg?L-1 IBA。在最佳培养基上，回归方程预测试管苗生根率高达82.28%；通过实际接种验证，生根率为80.5%，与理论值误差为2.16%。
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关键词:
雷公藤〓生根培养〓二次正交旋转组合
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DOI：
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分类号：
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基金项目：1.福建省大学生创新性实验计划项目(ZL0710/CS)；2.福建省高校服务海西重点建设项目（HX200801）；3.工信部中药材扶持项目（工信消费函［2011］119号）。 |
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Optimization of Rooting Culture Conditions of =Tripterygium wilfordii Hook.f. in Vitro |
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xingjianhong
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Abstract: |
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The aim of the study was to get the best condition of rooting culture of vitro for Tripterygium wilfordii Hook.f.. With the rooting rate for evaluation indexes，the optimum medium of rooting culture of vitro for T. wilfordii has been determined, through quadratic rotationorthogonal combination design for the concentration of the basic medium MS, IBA and ABT1#. 1/2MS 0.6 mg?L-1 ABT1# 0.2 mg?L-1 IBA were the best rooting culture medium. In the best culture medium the rooting rate of vitro as high as 82.28%. Through the actual inoculation test, the rooting rate is 80.5%, and the theoretical error was 2.16%.
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Key words:
Tripterygium wilfordii Hook.f., rooting induction, orthogonal design
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