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摘要: |
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利用发根农杆菌ATCC15834诱导金铁锁获得毛状根，研究金铁锁毛状根的最优培养条件。结果表明，在5种培养基中，以在1/2MS培养基中皂苷的总产量最大；添加3%蔗糖、水解乳蛋白(LH)及IBA利于毛状根的生长及总皂苷的积累；而添加IAA、NAA、6BA及蛋白胨抑制其生长。
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关键词:
金铁锁〓毛状根〓培养〓皂苷
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DOI：
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分类号：
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基金项目：林业公益性行业专项“华南立体林业经营模式研究与示范”（项目任务书编号：201004047）。 |
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Effects of Culture Conditions on the Hairy Roots Growth =of Psammosilene tunicoides |
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Wang Hongfeng
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Abstract: |
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Hairy root can be induced from Psammosilene tunicoides by 美高梅 美高梅 ATCC15834. The effects of different culture conditions on hairy roots growth were studied. Among five media used, the medium 1/2 MS was the best one for the saponins content. On the source concentration concerned, 3% sucrose concentration could increase the hairy root growth. The hydrolytic lactalbumin and IBA could improve the hairy root growth and total saponins production. IAA, NAA, 6BA and peptone could inhibit hairy root growth.
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Key words:
Psammosilene tunicoides， hairy roots， culture， saponins
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